IL-8 anticorps

N° du produit ABIN116091

Cet anticorps anti-IL-8 est un anticorps Lapin Polyclonal détectant IL-8 dans WB, IHC (p), IF, EIA et Func. Adapté pour Humain.

Aperçu rapide pour IL-8 anticorps (ABIN116091)

Antigène: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-8 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Functional Studies (Func)

Détail du produit anti-IL-8 anticorps

Purification: Affinity Chromatography employing immobilized hIL-8 matrix

Immunogène: Highly pure (>98%) E.coli derived recombinant Human Interleukin 8.

Information d'application

Indications d'application: Immunohistochemistry on Paraffin Sections1.0 μg/mL for two hours at room temperature or 0.25 μg/mL overnight at 4 °C. An HRPlabeled polymer detection system was used with a non-alcohol soluble AEC chromogenand a proteinase K antigen retrieval.
Optimal concentrations and conditions may vary. NeutralizationTo yield one-half maximal inhibition (ND50) of the biological activity of human IL-8 (100 ng/mL), a concentration of 2-3 μg/mL of this antibody is required. ELISAIndirect: To detect hIL-8 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hIL-8. Sandwich: To detect hIL-8 by sandwich ELISA (using 100 μL/well antibody solution) a

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur IL-8

Antigène: IL-8 (IL8) (Interleukin 8 (IL8))

Autre désignation: Interleukin-8 / IL8

Sujet: IL8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL8 attracts neutrophils, basophils, and T-cells, but not monocytes. Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology.Synonyms: CXCL8, Emoctakin, GCP1, Granulocyte chemotactic protein 1, MDNCF, MONAP, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAP1, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor

ID gène: 9606

UniProt: P10145

Pathways: Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy